The DetectX Serum Estradiol ELISA Kit is designed to quantitatively measure free 17β-estradiol present in serum and plasma samples. An estradiol standard is provided to generate a standard curve for the assay and all samples should be read from the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies.

An estradiol-peroxidase conjugate is added to the wells. The binding reaction for the EIA / ELISA is initiated by adding a sheep antibody to estradiol to each well. After incubation, the plate is washed and the substrate is added. The substrate reacts with the bound estradiol-peroxidase conjugate.

After a short incubation, the reaction is stopped and the intensity of the color generated by the EIA / ELISA is detected in a microtiter plate reader capable of measuring a wavelength of 450 nm.

17β-estradiol (E2) is a key regulator of growth, differentiation, and function in a wide range of issues, Estradiol ELISA Kit includes the male and female reproductive tracts, the mammary gland, the brain, the skeletal, and cardiovascular systems.

The predominant biological effects of E2 are mediated through two distinct intracellular receptors, ERa and ERß, each encoded by unique genes that possess the functional domain characteristics of the nuclear steroid/thyroid hormone receptor superfamily.

ERa is the predominant form that is expressed in the breast, uterus, cervix, and vagina. ERß exhibits a more limited pattern and is expressed primarily in the ovary, prostate, testis, spleen, lung, hypothalamus, and thymus. Estradiol also influences bone growth, brain development and maturation, and food intake, and is also critical to maintaining organ functions during severe trauma. In plasma, estradiol binds to serum proteins such as albumin and sex hormone transport globulin.

A little more than 2% of E2 is free and biologically active, the percentage remains constant throughout the menstrual cycle. Estradiol is conjugated in the liver with sulfate and glucuronide derivatives and is excreted. Inactivation includes conversion to less active estrogens, such as estrone and estriol. Estriol is the main urinary metabolite